PRP for Knee and Ligaments

My method of producing prp for early osteoarthritis knee, tennis elbow, mcl injury, rotator cuff injury and tendoachilis injury


Step 1 : - Collect a small quantity of blood in four syringes, the same is mixed with a small quantity of heparin(anticoagulant).

Step 2 :-  Centrifuge it at a certain speed to separate the RBC and Plasma.

Step 3 :-   Separate the components in two syringes.

Step 4 :-   Further mix the same with a medicine(inhibiting secondary) aggregation.

Step 5 :-   Centrifuge the syringe at a specific speed and specific time. on completion we have a separate PRP and Platelet poor plasma. Segregate the prp in single syringe.

Step 6 :-   Localise the lesion and prepare it aseptically. Inject the same at the injury site

Step 7 :-   Immobilise the lesion and allow it to heal for atleast 2 weeks.

Average Platelet concentration achieved by this method is about 7-8 times above the normal concentration in blood.

shailender      

Platelet rich plasma

PLATELET RICH PLASMA 

Platelet-rich plasma therapy involves collecting Blood(small quantity about 15 ml= two table spoons) from the patients arm. Followed by process of concentrating  platelets(approximately 2ml) from the whole blood and injecting them into the knee joint of the patient. 

WHAT IS PLATELET RICH PLASMA? (PRP)

Platelet Rich Plasma Therapy (PRP) is a promising new development in Sports Medicine. it helps in assisting and augmenting the natural healing ability of the human body.

It has been used for more than 10 years now. however its use in the management of osteoarthritis and tendon injuries has begin off late recently.

Potential uses for Platelet Rich Plasma Therapy (PRP) are:

• Early Osteoarthritis / cartilage wear(if early diagnosis of knee oa is made it can do wonders) e.g. knee pain

• Chronic tendon problems / Overuse injuries
  • e.g. tennis elbow, achilles 'tendinitis'

• Partial tendon tears not requiring surgery
  • e.g. Gluteal tendons, rotator cuff tendons

• Ligament and Muscle tears

Magic Cells - Platelets

Platelets - I call them Magic Cells -

                 Platelets are produced in Blood Cell Formation process in bone marrow. Normal Quantity of these cells in human body is 150-400 x 10 /micro lt. These magic cells live for only 5-9 days. They are stored in spleen and shot out when needed.

The main function of these magic cells in human body is to control bleeding when a blood vessel is damaged.

" Why and how are these magic cells"

" Platelets are a one of the natural sources of Growth Factors available to us at any age " 

                 Platelets contain Alpha and dense Granules. When a platelet is activated it excretes contents of these granules in the blood around it. "Alpha Granules contain "P-selectin, platelet factor 4, transforming growth factor-β1, platelet-derived growth factor, fibronectin, B-thromboglobulin,vWF, fibrinogen, and coagulation factors V and XIII".

What matters in Osteoarthritis is TGF beta -1(transforming growth factor) and PDGF(platelet derived growth factor).
" Documented and authenticated scientific research and 6 months regular followup following intraarticular infiltration of PDGF(PRGF- Platelet related growth factor) in patients with OA of the knee has shown improvement in function and quality of life. These favourable points allows us to consider this therapy as a treatment for OA"
Definately a good news for Osteoarthritic Patients- Regenerative medicine as a treatment modality for one of the most prevelant disease in the world.

Shailender